| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 311 | Coxiella burnetii | GCCAAACAATCGCGACCT | TCGCTGGCTTCTCGCTTT | 58.74 | 59.66 | 293 | 64 |
100.00%
|
50.00%
|
| Primer 312 | Coxiella burnetii | TGGACGCGTCGCATCTTT | TTGACGCAACGCACGGA | 60.05 | 60.26 | 185 | 64 |
100.00%
|
50.00%
|
| Primer 313 | Coxiella burnetii | TGCGCTGCTACTCCGATT | GCCCTTGATCCACGGCTAA | 59.11 | 59.78 | 182 | 63 |
100.00%
|
50.00%
|
| Primer 314 | Coxiella burnetii | ACGTAGCAGACAACAGCGG | GCACAACCACTGCATGCA | 60.37 | 59.27 | 165 | 63 |
100.00%
|
50.00%
|
| Primer 315 | Coxiella burnetii | TGCTGGCTGATGCGGTAT | TCGGCAGGCGAATGACTT | 59.09 | 59.34 | 217 | 63 |
100.00%
|
50.00%
|
| Primer 316 | Coxiella burnetii | TGCTGGCTGATGCGGTAT | TTCGGCAGGCGAATGACT | 59.09 | 59.34 | 218 | 63 |
100.00%
|
50.00%
|
| Primer 317 | Coxiella burnetii | ATGGATGCCGACACGCAA | CAATGATTCGCTGGCCTGC | 60.05 | 59.93 | 187 | 63 |
100.00%
|
50.00%
|
| Primer 318 | Coxiella burnetii | TCTTTGCTGGCCAAGGCA | TCGCAGGCTTTCCGTCAA | 59.80 | 59.58 | 294 | 63 |
100.00%
|
50.00%
|
| Primer 319 | Coxiella burnetii | GCTTGGGATTGCGCTCAA | ATTTCGCTCGACCGAGACC | 58.72 | 59.86 | 161 | 63 |
100.00%
|
50.00%
|
| Primer 320 | Coxiella burnetii | TGCTGCATTTGGAAGCGG | TGCGTGGATGGCAAAGGA | 59.04 | 59.57 | 174 | 63 |
100.00%
|
50.00%
|